Identification of a new population of Tnn+ progenitors to form tendon enthesis fibrocartilage
Elucidating the identity of enthesis-resident progenitors is critical for advancing regenerative strategies, particularly in the context of the long-standing question of how is fibrocartilage formed at tendon enthesis (bone-tendon interface) under mechanical loading. To address the question of cellu...
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| Autori principali: | , , , , , , |
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| Natura: | Article (Journal) |
| Lingua: | inglese |
| Pubblicazione: |
21 April 2026
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| In: |
Bone research
Year: 2026, Volume: 14, Fascicolo: 1, Pages: 1-14 |
| ISSN: | 2095-6231 |
| DOI: | 10.1038/s41413-026-00519-3 |
| Accesso online: | Verlag, kostenfrei, Volltext: https://doi.org/10.1038/s41413-026-00519-3 Verlag, kostenfrei, Volltext: https://www.nature.com/articles/s41413-026-00519-3 
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| Note sull'autore: | Tao Zhang, Lin Zhang, Ziyang Yuan, Linfeng Wang, Jianzhong Hu, Thomas Skutella and Hongbin Lu |
| Riassunto: | Elucidating the identity of enthesis-resident progenitors is critical for advancing regenerative strategies, particularly in the context of the long-standing question of how is fibrocartilage formed at tendon enthesis (bone-tendon interface) under mechanical loading. To address the question of cellular origins of entheseal fibrocartilage, we first employed spatial transcriptional and single cell sequencing to identify a novel population of Tnn⁺ progenitor cells and delineate their lineage trajectories across developmental stages. Subsequently, we used a diphtheria toxin mediated ablation model targeting these Tnn⁺ progenitors and demonstrated their functional importance, as ablation resulted in hypoplastic phenotypes characterized by impaired fibrocartilage maturation. Furthermore, comparative single-cell profiling between unloaded entheses and normal entheses revealed that tendon unloading significantly diminished both the abundance and chondrogenic potential of Tnn⁺ progenitors. Collectively, these findings resolve fundamental questions regarding enthesis morphogenesis and provide mechanistic insights into how mechanical loading orchestrates this critical developmental process. |
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| Descrizione del documento: | Im Titel ist das Pluszeichen bei Tnn+ hochgestellt Gesehen am 03.06.2026 |
| Descrizione fisica: | Online Resource |
| ISSN: | 2095-6231 |
| DOI: | 10.1038/s41413-026-00519-3 |