Novel paracellular marker based on ᴅ-dipeptide structure for highly sensitive quantification with UPLC-MS/MS for in vitro and in vivo blood-brain barrier permeability analysis
The blood-brain barrier (BBB) paracellular permeability must be evaluated in various contexts in vitro and in vivo, including pharmacological evaluation of drug candidates, investigations of pathological changes in disease and model development. However, most available paracellular marker substances...
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| Autori principali: | , , , , , , , |
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| Natura: | Article (Journal) |
| Lingua: | inglese |
| Pubblicazione: |
2026
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| In: |
Fluids and barriers of the CNS
Year: 2026, Volume: 23, Pages: 1-18 |
| ISSN: | 2045-8118 |
| DOI: | 10.1186/s12987-026-00806-5 |
| Accesso online: | Verlag, kostenfrei, Volltext: https://doi.org/10.1186/s12987-026-00806-5
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| Note sull'autore: | Cindy Bay, Eric Mühlberg, Philipp Uhl, Julia Carolin Stingl, Gzona Bajraktari-Sylejmani, Jürgen Burhenne, Johanna Weiss and Max Sauter |
| Riassunto: | The blood-brain barrier (BBB) paracellular permeability must be evaluated in various contexts in vitro and in vivo, including pharmacological evaluation of drug candidates, investigations of pathological changes in disease and model development. However, most available paracellular marker substances are either radioactive, lack sensitivity and thereby require large sample volumes, or they can influence the BBB themselves via osmotic pressure. Moreover, in drug permeability studies, an adequate paracellular marker should be detectable in the same sample as the compound being tested, ideally applying the same analytical technology. |
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| Descrizione del documento: | Online veröffentlicht: 10. April 2026 Gesehen am 15.06.2026 |
| Descrizione fisica: | Online Resource |
| ISSN: | 2045-8118 |
| DOI: | 10.1186/s12987-026-00806-5 |