Coiled-coil centrosomal proteins facilitate tubulin concentration via polyKR motifs
Centrosomes organize microtubules (MT) in animal cells by recruiting a group of MT polymerization factors to the pericentriolar matrix (PCM). Tubulin enrichment within the PCM contributes to microtubule-organizing activity. However, the molecular determinants underlying this tubulin concentration re...
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| Autori principali: | , , , , , , , , |
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| Natura: | Article (Journal) |
| Lingua: | inglese |
| Pubblicazione: |
03 April 2026
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| In: |
The FEBS journal
Year: 2026, Pages: 1-13 |
| ISSN: | 1742-4658 |
| DOI: | 10.1111/febs.70533 |
| Accesso online: | Resolving-System, lizenzpflichtig, Volltext: https://doi.org/10.1111/febs.70533 Verlag, lizenzpflichtig, Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1111/febs.70533 
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| Note sull'autore: | Shiou-Lan Lin, Yu-Hsun Chen, Ren Jun Loi, Yueh-Ting Chen, Ting Chen, Karine Lapouge, Garrett Greenan, Hans-Georg Kräusslich and Hui-Chun Cheng |
| Riassunto: | Centrosomes organize microtubules (MT) in animal cells by recruiting a group of MT polymerization factors to the pericentriolar matrix (PCM). Tubulin enrichment within the PCM contributes to microtubule-organizing activity. However, the molecular determinants underlying this tubulin concentration remain elusive. Here, we describe a mechanism of tubulin concentration by polyKR motifs encoded in the centrosomal coiled-coil proteins. We show that polyKR sequences promote tubulin enrichment within engineered protein condensates that mimic the protein-dense environment of the centrosome. Bioinformatic analysis reveals that polyKR motif-containing coiled-coil proteins are highly enriched in centrosomes across species, from worms and flies to mice and humans. The tubulin binding affinity of polyKR motifs with six to 10 basic residues is approximately in the micromolar to submicromolar range. Cytoplasmic puncta containing the predicted centrosomal polyKR motifs concentrate microtubules. In purified systems, we show that TACC3 and Cep63 condensates containing polyKR motifs concentrate tubulin dimers over 10-fold. Moreover, the spatial density of polyKR motifs correlates with MT concentration, suggesting that cells can modulate tubulin concentration at the PCM by tuning the centrosomal composition. Our findings highlight a general function encoded in centrosomal scaffolding proteins for tubulin concentration, and this mechanism is functionally redundant to ensure robust tubulin enrichment in the PCM. |
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| Descrizione del documento: | Online version of record before inclusion in an issue Gesehen am 23.06.2026 |
| Descrizione fisica: | Online Resource |
| ISSN: | 1742-4658 |
| DOI: | 10.1111/febs.70533 |