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A luciferase-based hRBC-NSG mouse model to evaluate anti-CelTOS transmission-blocking antibodies against P. falciparum

Reliable experimental platforms are essential for evaluating transmission-blocking interventions against Plasmodium falciparum (P. falciparum). Although the standard membrane feeding assay (SMFA) is widely used to assess transmission-blocking activity, it is performed in vitro and does not fully rep...

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Main Authors: Xu, Shulin (Author) , Roth, Alison E. (Author) , Thomson-Luque, Richard (Author) , Salinas, Nichole (Author) , Barnes, Samantha (Author) , Herman, Courtney (Author) , Tolia, Niraj H. (Author) , Adams, John H. (Author)
Format: Article (Journal)
Language:English
Published: 01 April 2026
In: Malaria journal
Year: 2026, Volume: 25, Pages: 1-14
ISSN:1475-2875
DOI:10.1186/s12936-026-05894-0
Online Access:Verlag, kostenfrei, Volltext: https://doi.org/10.1186/s12936-026-05894-0
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Author Notes:Shulin Xu, Alison E. Roth, Richard Thomson-Luque, Nichole Salinas, Samantha Barnes, Courtney Herman, Niraj H. Tolia and John H. Adams
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Summary:Reliable experimental platforms are essential for evaluating transmission-blocking interventions against Plasmodium falciparum (P. falciparum). Although the standard membrane feeding assay (SMFA) is widely used to assess transmission-blocking activity, it is performed in vitro and does not fully represent physiological conditions. In this study, we developed and applied a luciferase-based assay using NSG (NOD.Cg-Prkdc^scid Il2rg^tm1Sug/JicTac) mice engrafted with human red blood cells (hRBCs) to establish an hRBC-engrafted NSG (hRBC-NSG) humanized mouse model for evaluating the transmission-blocking activity of anti-CelTOS monoclonal antibodies (mAbs) against P. falciparum.
Item Description:Gesehen am 06.07.2026
Physical Description:Online Resource
ISSN:1475-2875
DOI:10.1186/s12936-026-05894-0

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