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Plant heteropolysaccharides and their bioadhesion to animal glycocalyx visualized at the nanoscale [data]

The plant-derived biopolymer pectin has been explored as sealant in lung surgery. Interpenetration of pectin’s heteropolysaccharide chains with sugar-chains on components of the mesothelial glycocalyx is thought to mediate adhesion. To visualize a potential adhesion complex, we first established a m...

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Dettagli Bibliografici
Autori principali: Wacker, Irene (Autore) , Wagner, Willi Linus (Autore) , Curticean, Ernest Ronald (Autore) , Schoch, Anna (Autore) , Majorovits, Endre (Autore) , Pan, Jennifer M. (Autore) , Mentzer, Steven J. (Autore) , Bunzel, Mirko (Autore) , Schröder, Rasmus R. (Autore)
Natura: Database Research Data
Lingua:inglese
Pubblicazione: Heidelberg Universität 2026-07-14
DOI:10.11588/DATA/5LW3JS
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Forschungsdaten
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Accesso online:Verlag, kostenfrei, Volltext: https://doi.org/10.11588/DATA/5LW3JS
Verlag, kostenfrei, Volltext: https://heidata.uni-heidelberg.de/dataset.xhtml?persistentId=doi:10.11588/DATA/5LW3JS
Testo
Note sull'autore:Irene Wacker, Willi Wagner, Ronald Curticean, Anna Schoch, Endre Majorovits, Jennifer M. Pan, Steven J. Mentzer, Mirko Bunzel, Rasmus R. Schröder
Descrizione
Riassunto:The plant-derived biopolymer pectin has been explored as sealant in lung surgery. Interpenetration of pectin’s heteropolysaccharide chains with sugar-chains on components of the mesothelial glycocalyx is thought to mediate adhesion. To visualize a potential adhesion complex, we first established a method to preserve pectin ultrastructure by limiting hydration during sample preparation for electron microscopy. Depending on actual hydration states and pectic domains selected we find distinct morphologies: Citrus pectic polysaccharides, combining both, smooth homogalacturonan and hairy rhamnogalacturonan domains, form a loose network of thin filaments. Rhamnogalacturonan and xylogalacturonan domains from soy show a denser mesh, while pectic galactan from potato or pectic arabinan from sugar beet display more globular aspects. As proof of concept, we finally applied the filamentous lemon pectin to the surface of murine lung in a surgical setting. Ultrastructural visualization of the sealant-tissue assembly shows tight association between the carbohydrate fibers, possibly driven by polymer-typical chain interactions.
Descrizione del documento:Gefördert durch: Deutsche Forschungsgemeinschaft: Excellence Cluster ‘3D Matter Made to Order’ EXC-2082/1 – 390761711; Bundeministerium Bildung und Forschung: project NanoPatho, FKZ 13N14476; Deutsche Forschungsgemeinschaft: INST 35/1314-1 FUGG; Deutsche Forschungsgemeinschaft: INST 35/1503-1 FUGG
Gesehen am 15.07.2026
Descrizione fisica:Online Resource
DOI:10.11588/DATA/5LW3JS

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