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Role of bacterial and host DNases on host-pathogen interaction during Streptococcus suis Meningitis

Streptococcus suis is a zoonotic agent causing meningitis in pigs and humans. Neutrophils, as the first line of defense against S. suis infections, release neutrophil extracellular traps (NETs) to entrap pathogens. In this study, we investigated the role of the secreted nuclease A of S. suis (SsnA)...

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Autori principali: Meurer, Marita (Autore) , Öhlmann, Sophie (Autore) , Bonilla Gonzalez, Marta Cristina (Autore) , Valentin-Weigand, Peter (Autore) , Beineke, Andreas (Autore) , Hennig-Pauka, Isabel (Autore) , Schwerk, Christian (Autore) , Schroten, Horst (Autore) , Baums, Christoph (Autore) , Köckritz-Blickwede, Maren von (Autore) , Buhr, Nicole de (Autore)
Natura: Article (Journal)
Lingua:inglese
Pubblicazione: 2020
In: International journal of molecular sciences
Year: 2020, Volume: 21, Fascicolo: 15, Pages: 1-21
ISSN:1422-0067
DOI:10.3390/ijms21155289
Accesso online:Verlag, kostenfrei, Volltext: https://doi.org/10.3390/ijms21155289
Verlag, kostenfrei, Volltext: https://www.mdpi.com/1422-0067/21/15/5289
Testo
Note sull'autore:Marita Meurer, Sophie Öhlmann, Marta C. Bonilla, Peter Valentin-Weigand, Andreas Beineke, Isabel Hennig-Pauka, Christian Schwerk, Horst Schroten, Christoph G. Baums, Maren von Köckritz-Blickwede and Nicole de Buhr
Descrizione
Riassunto:Streptococcus suis is a zoonotic agent causing meningitis in pigs and humans. Neutrophils, as the first line of defense against S. suis infections, release neutrophil extracellular traps (NETs) to entrap pathogens. In this study, we investigated the role of the secreted nuclease A of S. suis (SsnA) as a NET-evasion factor in vivo and in vitro. Piglets were intranasally infected with S. suis strain 10 or an isogenic ssnA mutant. DNase and NET-formation were analyzed in cerebrospinal fluid (CSF) and brain tissue. Animals infected with S. suis strain 10 or S. suis 10ΔssnA showed the presence of NETs in CSF and developed similar clinical signs. Therefore, SsnA does not seem to be a crucial virulence factor that contributes to the development of meningitis in pigs. Importantly, DNase activity was detectable in the CSF of both infection groups, indicating that host nucleases, in contrast to bacterial nuclease SsnA, may play a major role during the onset of meningitis. The effect of DNase 1 on neutrophil functions was further analyzed in a 3D-cell culture model of the porcine blood-CSF barrier. We found that DNase 1 partially contributes to enhanced killing of S. suis by neutrophils, especially when plasma is present. In summary, host nucleases may partially contribute to efficient innate immune response in the CSF.
Descrizione del documento:Gesehen am 23.07.2026
Descrizione fisica:Online Resource
ISSN:1422-0067
DOI:10.3390/ijms21155289

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