Increased carcinoembryonic antigen expression on the surface of lung cancer cells using gold nanoparticles during radiotherapy
Purpose - Tumor-associated antigens are a promising target of immunotherapy approaches for cancer treatments but rely on sufficient expression of the target antigen. This study investigates the expression of the carcinoembryonic antigen (CEA) on the surface of irradiated lung cancer cells in vitro u...
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| Autori principali: | , , , , , , , |
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| Natura: | Article (Journal) |
| Lingua: | inglese |
| Pubblicazione: |
August 2020
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| In: |
Physica medica
Year: 2020, Volume: 76, Pages: 236-242 |
| ISSN: | 1724-191X |
| DOI: | 10.1016/j.ejmp.2020.06.024 |
| Accesso online: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1016/j.ejmp.2020.06.024 Verlag, lizenzpflichtig, Volltext: https://www.sciencedirect.com/science/article/pii/S1120179720301587 
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| Note sull'autore: | Romy Mueller, Sayeda Yasmin-Karim, Kaylie DeCosmo, Ana Vazquez-Pagan, Srinivas Sridhar, David Kozono, Juergen Hesser, Wilfred Ngwa |
| Riassunto: | Purpose - Tumor-associated antigens are a promising target of immunotherapy approaches for cancer treatments but rely on sufficient expression of the target antigen. This study investigates the expression of the carcinoembryonic antigen (CEA) on the surface of irradiated lung cancer cells in vitro using gold nanoparticles as radio-enhancer. - Methods - Human lung carcinoma cells A549 were irradiated and expression of CEA on the cell surface measured by flow cytometry 3 h, 24 h, and 72 h after irradiation to doses of 2 Gy, 6 Gy, 10 Gy, and 20 Gy in the presence or absence of 0.1 mg/ml or 0.5 mg/ml gold nanoparticles. CEA expression was measured as median fluorescent intensity and percentage of CEA-positive cells. - Results - An increase in CEA expression was observed with both increasing radiation dose and time. There was doubling in median fluorescent intensity 24 h after 20 Gy irradiation and 72 h after 6 Gy irradiation. Use of gold nanoparticles resulted in additional significant increase in CEA expression. Change in cell morphology included swelling of cells and increased internal complexity in accordance with change in CEA expression. - Conclusions - This study showed an increase in CEA expression on human lung carcinoma cells following irradiation. Increase in expression was observed with increasing radiation dose and in a time dependent manner up to 72 h post irradiation. The results further showed that gold nanoparticles can significantly increase CEA expression following radiotherapy. |
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| Descrizione del documento: | Gesehen am 27.07.2026 |
| Descrizione fisica: | Online Resource |
| ISSN: | 1724-191X |
| DOI: | 10.1016/j.ejmp.2020.06.024 |