HIV-induced IL-6/IL-10 dysregulation of CD4 cells is associated with defective B cell help and autoantibody formation against CD4 cells
To analyse CD4 cell cytokine secretion and helper/suppressor function at a clonal level we established 446 CD4+ T cell clones (TCC) in four healthy controls, three HIV− haemophilia patients, four CDC II,III and four CDC IV patients. Spontaneous TCC secretion of Th1 cytokines (IL-2, interferon-gamma...
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| Auteurs principaux: | , , , , , |
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| Format: | Article (Journal) |
| Langue: | anglais |
| Publié: |
January 1998
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| In: |
Clinical & experimental immunology
Year: 1998, Volume: 111, Numéro: 1, Pages: 20-29 |
| ISSN: | 1365-2249 |
| DOI: | 10.1046/j.1365-2249.1998.00429.x |
| Accès en ligne: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1046/j.1365-2249.1998.00429.x Verlag, lizenzpflichtig, Volltext: https://pmc.ncbi.nlm.nih.gov/articles/PMC1904842/ 
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| Notes sur l'auteur: | R. Weimer, S. Zipperle, V. Daniel, R. Zimmermann, K. Schimpf & G. Opelz |
| Résumé: | To analyse CD4 cell cytokine secretion and helper/suppressor function at a clonal level we established 446 CD4+ T cell clones (TCC) in four healthy controls, three HIV− haemophilia patients, four CDC II,III and four CDC IV patients. Spontaneous TCC secretion of Th1 cytokines (IL-2, interferon-gamma (IFN-γ)) and Th2 cytokines (IL-4, IL-6, IL-10) was determined by ELISA. TCC helper and suppressor functions were tested in a pokeweed mitogen (PWM)-stimulated allogeneic co-culture system using a reverse haemolytic plaque assay for assessment of B cell responses. There was a significant association of TCC surface marker expression (Leu-8, CD45RA) with TCC IL-6 secretion in healthy controls (P < 0·01), HIV− patients (P ≤ 0·001) and CDC II,III patients (P ≤ 0·01) but not in CDC IV patients. Likewise, TCC expression of Leu-8 and CD45RA was significantly associated with TCC suppressor function in healthy controls (P ≤ 0·0005) but not in HIV-infected patients. A reduced TCC helper frequency (≤ 10% of TCC) and an enhanced TCC suppressor frequency (> 80% of TCC) were detected only in those HIV-infected patients who showed an excessively increased TCC IL-6 secretion (> 70% of TCC) together with a significantly diminished TCC IL-10 secretion (≤ 10% of TCC). CD4 cell autoantibodies also were found only in patients with this type of cytokine dysregulation. These data indicate that CD4 cell surface markers lose their functional relevance in HIV-infected patients. HIV-induced IL-6/IL-10 dysregulation of CD4+ T cells, i.e. the up-regulation of spontaneous IL-6 and down-regulation of spontaneous IL-10 secretion, appears to be involved in inducing CD4 helper defects and may promote autoantibody formation against CD4 cells. |
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| Description: | Online veröffentlicht: 20. Oktober 2008 Gesehen am 25.08.2026 |
| Description matérielle: | Online Resource |
| ISSN: | 1365-2249 |
| DOI: | 10.1046/j.1365-2249.1998.00429.x |