Peroxisomal protein import In vivo evidence for a novel ranslocation competent compartment
In homogenates of isolated hepatocytes separated by Nycodenz density gradient centrifugation, two peroxisomal populations are identified that differ in buoyant density. Organelles present in a high density fraction (1.22-1.23 g/cm3) represent mature peroxisomes. Vesicles of intermediate density (1.1...
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| Autori principali: | , |
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| Natura: | Article (Journal) |
| Lingua: | inglese |
| Pubblicazione: |
March 30, 1992
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| In: |
FEBS letters
Year: 1992, Volume: 300, Fascicolo: 2, Pages: 179-182 |
| ISSN: | 1873-3468 |
| DOI: | 10.1016/0014-5793(92)80191-I |
| Accesso online: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1016/0014-5793(92)80191-I Verlag, lizenzpflichtig, Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1016/0014-5793%2892%2980191-I 
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| Note sull'autore: | Petra Heinemann, Wilhelm W. Just |
| Riassunto: | In homogenates of isolated hepatocytes separated by Nycodenz density gradient centrifugation, two peroxisomal populations are identified that differ in buoyant density. Organelles present in a high density fraction (1.22-1.23 g/cm3) represent mature peroxisomes. Vesicles of intermediate density (1.16-1.17 gm̧3) represent mature peroxisomes. Vesicles of intermediate density (1.16-1.17 g/cm3) are present in much lower concentration and seem to play a particular role in the import and distribution or newly synthesized peroxisomal proteins. In a typical pulse-chase experiment with a 7.5 min pulse, peroxisomal acyl-CoA oxidase is first imported into the peroxisomal fraction of intermediate density. After a chase of up to 30 min, the enzyme is found in mature peroxisomes. |
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| Descrizione del documento: | Gesehen am 26.08.2026 |
| Descrizione fisica: | Online Resource |
| ISSN: | 1873-3468 |
| DOI: | 10.1016/0014-5793(92)80191-I |