Defective IL-6 secretion in HIV-infected haemophilia patients
To study the role of IL-6 in HIV-induced B cell defects, in vitro B cell responses and IL-6 secretion were determined simultaneously in 67 haemophilia patients, Twenty-three palients were HIV (Group 1). 27 HIV+ stage CDC II, III (Group 2). and 17 vi-ere HIV4 stage CDC IV (Group 3). Pokeweed mitogen...
Guardado en:
| Autores principales: | , , , , |
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| Formato: | Article (Journal) |
| Lenguaje: | inglés |
| Publicado: |
February 1993
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| In: |
Clinical & experimental immunology
Year: 1993, Volumen: 91, Número: 2, Pages: 249-256 |
| ISSN: | 1365-2249 |
| DOI: | 10.1111/j.1365-2249.1993.tb05891.x |
| Acceso en línea: | Resolving-System, lizenzpflichtig, Volltext: https://doi.org/10.1111/j.1365-2249.1993.tb05891.x Verlag, lizenzpflichtig, Volltext: https://academic.oup.com/cei/article-abstract/91/2/249/6487811?redirectedFrom=fulltext&login=true 
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| Notas de Autor: | R. Weimer, S. Zipperle, V. Daniel, R. Zimmermann, G. Opelz |
| Sumario: | To study the role of IL-6 in HIV-induced B cell defects, in vitro B cell responses and IL-6 secretion were determined simultaneously in 67 haemophilia patients, Twenty-three palients were HIV (Group 1). 27 HIV+ stage CDC II, III (Group 2). and 17 vi-ere HIV4 stage CDC IV (Group 3). Pokeweed mitogen (PWM) was used for TeelI-dependent and Staphylococcus aureus Cowan 1 (SAC I) for T cell-independent B cell stimulalion. B cell differentiation was assessed in a reverse haemolytic plaque assay and by ELISA determination of IgG and IgM in culture supernatants. An ELISA was used to measure IL-6 in plasma and culture supernatants, HIV+ patients showed impaired immunoglobulin-secreting cell (ISC) responses after T cell-independent and T cell-dependent stimulation (P < 0·000l and P<0·01, respectively), whereas IL-6 secretion. IgM and IgG responses were comparable to those in hcallhy controls. HIV+ patients at stage CDC II, Ml or IV demonst rated significantly reduced mitogen-stimulated IL-6 secretion (P < 0·05. PWM; P < 0·001. SAC lj as well as impaired ISC and IgG responses (P < 0·01. PWM; P≤ 0·0001, SAC I), CDC IV patients showed reduced IgM responses in addition (P < 0·02, PWM; P < 0·0005. SAC I). Plasma iL-6 levels were elevated both in HIV+ patients (CDC II, III patients: 165 ± 73 pg/ml. P < 0·005; CDC IV patients: 58 ± 18 pg/ml, P < 0·001) and in HIV patients (283 ± 65 pg/ ml. P<0·0001) which appeared to be a T cell effect induced by treatment with haemophilia factor concentrates. Our data provide evidence for different types of B cell deficiencies in HIV patients (impaired ISC response only) and HIV+ patients (impaired ISC as well as IL-6 and IgM /lgG responses). The defective IL-6 secretion in HIV+ patients is likely to affect terminal B cell differentiation and this may explain the reduced immunoglobulin secretion in these patients in response to antigenic challenge. |
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| Notas: | Online veröffentlicht: 28. Juni 2008 Gesehen am 26.08.2026 |
| Descripción Física: | Online Resource |
| ISSN: | 1365-2249 |
| DOI: | 10.1111/j.1365-2249.1993.tb05891.x |