Brillouin spectroscopy reveals mechanical properties beyond hydration
Brillouin light scattering is a noncontact technique for probing the micromechanical properties of cells and tissues through the GHz-frequency longitudinal modulus. Interpreting Brillouin spectra in hydrated biological materials remains challenging because the Brillouin shift is influenced not only...
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| Autores principales: | , , , , , |
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| Formato: | Article (Journal) Chapter/Article |
| Lenguaje: | inglés |
| Publicado: |
1 Sep 2026
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| Edición: | Version v2 |
| In: |
Arxiv
Year: 2026, Pages: 1-21 |
| DOI: | 10.48550/arXiv.2602.08150 |
| Acceso en línea: | Verlag, kostenfrei, Volltext: https://doi.org/10.48550/arXiv.2602.08150 Verlag, kostenfrei, Volltext: http://arxiv.org/abs/2602.08150 
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| Notas de Autor: | Zhe Wang, Maria Regato Herbella, Fereydoon Taheri, Maria de los Angeles De la Cruz Garcia, Gaurav Dave, and Christine Selhuber-Unkel |
| Sumario: | Brillouin light scattering is a noncontact technique for probing the micromechanical properties of cells and tissues through the GHz-frequency longitudinal modulus. Interpreting Brillouin spectra in hydrated biological materials remains challenging because the Brillouin shift is influenced not only by water content but also by polymer network mechanics, relaxation processes, and viscous dissipation. Here, we investigated two model hydrogels with distinct network chemistry, together with a binary solvent mixture, to disentangle these contributions. Time- and space-resolved measurements of a hydrating hydrogel further show that Brillouin microscopy captures local mechanical changes that bulk rheometry only partially resolves. Experiments on ethanol-water mixtures further demonstrate that the Brillouin shift does not vary monotonically with hydration but instead reflects changes in the mixture's mechanical properties. In addition, by comparing longitudinal, bulk, and shear viscosities, we identify bulk viscous dissipation as an important contributor to the Brillouin response. Together, these results show that hydration modulates Brillouin spectra but does not fully determine them. Our findings provide a mechanical framework for interpreting Brillouin measurements in hydrated and biomolecular systems. |
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| Notas: | Gesehen am 02.09.2026 |
| Descripción Física: | Online Resource |
| DOI: | 10.48550/arXiv.2602.08150 |