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Mass spectrometric proteome profiling using a deep spectral library reveals homogenization of right and left atrial proteomes in persistent atrial fibrillation patients

Aims Pulmonary veins in the left atrium (LA) are well-established as a critical site for the initiation of atrial fibrillation (AF). Emerging evidence suggests that in persistent AF (persAF), AF triggers may extend beyond LA. However, the extent to which AF-associated remodelling involves the right...

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Autori principali: Liutkute, Aiste (Autore) , Berrandou, Takiy-Eddine (Autore) , Kestel, Stefanie (Autore) , Schnelle, Moritz (Autore) , Dschun, Olga (Autore) , Amedei, Hugo Alejandro (Autore) , Neuenroth, Lisa (Autore) , Rytkin, Eric (Autore) , Kyshynska, Oksana (Autore) , Kensah, George (Autore) , El-Essawi, Aschraf (Autore) , Jebran, Ahmad Fawad (Autore) , Danner, Bernhard C. (Autore) , Baraki, Hassina (Autore) , Kutschka, Ingo (Autore) , Bremmer, Felix (Autore) , Urlaub, Henning (Autore) , Schmidt, Constanze (Autore) , Bouatia-Naji, Nabila (Autore) , Efimov, Igor R. (Autore) , Brundel, Bianca J. J. M. (Autore) , Lenz, Christof (Autore) , Voigt, Niels (Autore)
Natura: Article (Journal)
Lingua:inglese
Pubblicazione: May 2026
In: Cardiovascular research
Year: 2026, Volume: 122, Fascicolo: 8, Pages: 1037-1054
ISSN:1755-3245
DOI:10.1093/cvr/cvag076
Accesso online:Verlag, kostenfrei, Volltext: https://doi.org/10.1093/cvr/cvag076
Testo
Note sull'autore:Aiste Liutkute, Takiy-Eddine Berrandou, Stefanie Kestel, Moritz Schnelle, Olga Dschun, Hugo Alejandro Amedei, Lisa Neuenroth, Eric Rytkin, Oksana Kyshynska, George Kensah, Aschraf El-Essawi, Ahmad Fawad Jebran, Bernhard C. Danner, Hassina Baraki, Ingo Kutschka, Felix Bremmer, Henning Urlaub, Constanze Schmidt, Nabila Bouatia-Naji, Igor R. Efimov, Bianca J.J.M. Brundel, Christof Lenz, and Niels Voigt
Descrizione
Riassunto:Aims Pulmonary veins in the left atrium (LA) are well-established as a critical site for the initiation of atrial fibrillation (AF). Emerging evidence suggests that in persistent AF (persAF), AF triggers may extend beyond LA. However, the extent to which AF-associated remodelling involves the right atrium (RA) in persAF remains a subject of debate. To address this, we employed a proteomic approach aiming at investigating AF-associated remodelling in the RA relative to the LA in persAF.Methods and results RA and LA samples were obtained from sinus rhythm (SR) patients, patients with persAF undergoing open-heart surgery, and non-failing donor hearts rejected for transplantation. A reference spectral library representing the human cardiac proteome was employed to assess the RA and LA proteomes by data-independent acquisition mass spectrometry. Protein levels were quantified by immunoblotting of human atrial tissue. Plasma levels of NT-proANP and NT-proBNP were measured in SR and persAF patients. Fibrosis levels were quantified in paraffin-embedded sections using Masson-Goldner trichrome staining. A spectral library representing 13 539 human proteins was generated from five anatomical regions of five independent donor hearts. In persAF RA, we observed marked myolysis, excessive extracellular matrix deposition and a prominent similarity to the failing ventricular proteome, all comparable to persAF LA. Although significant proteomic differences were observed between the RA and LA from SR patients, a comparison of RA and LA proteomes in persAF patients revealed proteome homogenization between the two atrial chambers. RA contributes to this homogenization by losing RA-specific markers, while gaining LA-specific markers.Conclusion Our findings suggest that RA undergoes comparable AF-associated remodelling to LA, contributing to atrial proteome unification which represents a hallmark of persAF.
Descrizione del documento:Veröffentlicht: 2 April 2026
Gesehen am 21.09.2026
Descrizione fisica:Online Resource
ISSN:1755-3245
DOI:10.1093/cvr/cvag076

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