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Mass spectrometric proteome profiling using a deep spectral library reveals homogenization of right and left atrial proteomes in persistent atrial fibrillation patients

Aims Pulmonary veins in the left atrium (LA) are well-established as a critical site for the initiation of atrial fibrillation (AF). Emerging evidence suggests that in persistent AF (persAF), AF triggers may extend beyond LA. However, the extent to which AF-associated remodelling involves the right...

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Main Authors: Liutkute, Aiste (Author) , Berrandou, Takiy-Eddine (Author) , Kestel, Stefanie (Author) , Schnelle, Moritz (Author) , Dschun, Olga (Author) , Amedei, Hugo Alejandro (Author) , Neuenroth, Lisa (Author) , Rytkin, Eric (Author) , Kyshynska, Oksana (Author) , Kensah, George (Author) , El-Essawi, Aschraf (Author) , Jebran, Ahmad Fawad (Author) , Danner, Bernhard C. (Author) , Baraki, Hassina (Author) , Kutschka, Ingo (Author) , Bremmer, Felix (Author) , Urlaub, Henning (Author) , Schmidt, Constanze (Author) , Bouatia-Naji, Nabila (Author) , Efimov, Igor R. (Author) , Brundel, Bianca J. J. M. (Author) , Lenz, Christof (Author) , Voigt, Niels (Author)
Format: Article (Journal)
Language:English
Published: May 2026
In: Cardiovascular research
Year: 2026, Volume: 122, Issue: 8, Pages: 1037-1054
ISSN:1755-3245
DOI:10.1093/cvr/cvag076
Online Access:Verlag, kostenfrei, Volltext: https://doi.org/10.1093/cvr/cvag076
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Author Notes:Aiste Liutkute, Takiy-Eddine Berrandou, Stefanie Kestel, Moritz Schnelle, Olga Dschun, Hugo Alejandro Amedei, Lisa Neuenroth, Eric Rytkin, Oksana Kyshynska, George Kensah, Aschraf El-Essawi, Ahmad Fawad Jebran, Bernhard C. Danner, Hassina Baraki, Ingo Kutschka, Felix Bremmer, Henning Urlaub, Constanze Schmidt, Nabila Bouatia-Naji, Igor R. Efimov, Bianca J.J.M. Brundel, Christof Lenz, and Niels Voigt
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Summary:Aims Pulmonary veins in the left atrium (LA) are well-established as a critical site for the initiation of atrial fibrillation (AF). Emerging evidence suggests that in persistent AF (persAF), AF triggers may extend beyond LA. However, the extent to which AF-associated remodelling involves the right atrium (RA) in persAF remains a subject of debate. To address this, we employed a proteomic approach aiming at investigating AF-associated remodelling in the RA relative to the LA in persAF.Methods and results RA and LA samples were obtained from sinus rhythm (SR) patients, patients with persAF undergoing open-heart surgery, and non-failing donor hearts rejected for transplantation. A reference spectral library representing the human cardiac proteome was employed to assess the RA and LA proteomes by data-independent acquisition mass spectrometry. Protein levels were quantified by immunoblotting of human atrial tissue. Plasma levels of NT-proANP and NT-proBNP were measured in SR and persAF patients. Fibrosis levels were quantified in paraffin-embedded sections using Masson-Goldner trichrome staining. A spectral library representing 13 539 human proteins was generated from five anatomical regions of five independent donor hearts. In persAF RA, we observed marked myolysis, excessive extracellular matrix deposition and a prominent similarity to the failing ventricular proteome, all comparable to persAF LA. Although significant proteomic differences were observed between the RA and LA from SR patients, a comparison of RA and LA proteomes in persAF patients revealed proteome homogenization between the two atrial chambers. RA contributes to this homogenization by losing RA-specific markers, while gaining LA-specific markers.Conclusion Our findings suggest that RA undergoes comparable AF-associated remodelling to LA, contributing to atrial proteome unification which represents a hallmark of persAF.
Item Description:Veröffentlicht: 2 April 2026
Gesehen am 21.09.2026
Physical Description:Online Resource
ISSN:1755-3245
DOI:10.1093/cvr/cvag076

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