DNA origami signaling inits transduce chemical and mechanical signals in synthetic cells
Transmembrane proteins transmit chemical signals as well as mechanical cues. The latter is often achieved by coupling to the cytoskeleton. The incorporation of fully engineerable membrane-spanning structures for the transduction of chemical and, in particular, mechanical signals is therefore a criti...
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| Autori principali: | , , , , , |
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| Natura: | Article (Journal) |
| Lingua: | inglese |
| Pubblicazione: |
May 16, 2024
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| In: |
Advanced functional materials
Year: 2024, Volume: 34, Fascicolo: 20, Pages: 1-10 |
| ISSN: | 1616-3028 |
| DOI: | 10.1002/adfm.202301176 |
| Accesso online: | Verlag, kostenfrei, Volltext: https://doi.org/10.1002/adfm.202301176 Verlag, kostenfrei, Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1002/adfm.202301176 
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| Note sull'autore: | Kevin Jahnke, Maja Illig, Marlene Scheffold, Mai P. Tran, Ulrike Mersdorf, and Kerstin Göpfrich |
| Riassunto: | Transmembrane proteins transmit chemical signals as well as mechanical cues. The latter is often achieved by coupling to the cytoskeleton. The incorporation of fully engineerable membrane-spanning structures for the transduction of chemical and, in particular, mechanical signals is therefore a critical aim for bottom-up synthetic biology. Here, a membrane-spanning DNA origami signaling units (DOSUs) is designed and mechanically coupled to DNA cytoskeletons encapsulated within giant unilamellar vesicles (GUVs). The incorporation of the DOSUs into the GUV membranes is verified and clustering upon external stimulation is achieved. Dye-influx assays reveal that clustering increases the insertion efficiency. The transmembrane-spanning DOSUs act as pores to allow for the transport of single-stranded DNA into the GUVs. This is employed to trigger the reconfiguration of DNA cytoskeletons within GUVs. In addition to chemical signaling, mechanical coupling of the DOSUs to the internal DNA cytoskeletons is induced. With chemical cues from the environment, clustering of the DOSUs is induced, which triggers a symmetry break in the organization of the DNA cytoskeleton inside of the GUV. DNA-based transmembrane structures are engineered that transduce signals without transporting the signaling molecule itself—providing a route toward signal processing and adaptive synthetic cells. |
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| Descrizione del documento: | Gesehen am 24.09.2026 |
| Descrizione fisica: | Online Resource |
| ISSN: | 1616-3028 |
| DOI: | 10.1002/adfm.202301176 |