Identification and characterization of thermostable uracil glycosylases from the archaeon Methanobacterium thermoautotrophicum and the bacterium Thermus thermophilus
Hydrolytic deamination reactions of cytosine and 5-methylcytosine results in highly mutagenic U/G and T/G mismatches, respectively. These processes are especially pronounced at high temperatures and present serious threats to genomic integrity for thermophiles. It is believed, that the respective da...
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| Dokumenttyp: | Book/Monograph Hochschulschrift |
| Sprache: | Englisch |
| Veröffentlicht: |
2001
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| Ausgabe: | Electronic edition |
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| Online-Zugang: | Verlag, Volltext: http://webdoc.sub.gwdg.de/diss/2002/starkuviene/index.html Resolving-System, Volltext: http://nbn-resolving.de/urn:nbn:de:gbv:7-webdoc-1238-1 
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| Verfasserangaben: | von Vytaute Starkuviene |
| Zusammenfassung: | Hydrolytic deamination reactions of cytosine and 5-methylcytosine results in highly mutagenic U/G and T/G mismatches, respectively. These processes are especially pronounced at high temperatures and present serious threats to genomic integrity for thermophiles. It is believed, that the respective damages are repaired by the base excision machinery (BER) which involves the action of uracil and T/G, U/G mismatches specific DNA glycosylases. With the aim to deepen knowledge about U/G and T/G removing enzymes, the focus of this work was done on isolation and enzymatic characterisation of several novel uracil glycosylases from two thermophilic microorganisms: archaeon Methanobacterium thermoautotrophicum DH and bacteria Thermus thermophilus HB27. In vivo role of characterised proteins was discussed by combining the enzymatic data with the comparative genomic analysis |
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| Beschreibung: | Online Resource |