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Cell adhesion strength is controlled by intermolecular spacing of adhesion receptors

Spatial patterning of biochemical cues on the micro- and nanometer scale controls numerous cellular processes such as spreading, adhesion, migration, and proliferation. Using force microscopy we show that the lateral spacing of individual integrin receptor-ligand bonds determines the strength of cel...

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Bibliographic Details
Main Authors: Selhuber-Unkel, Christine (Author) , Erdmann, Thorsten (Author) , Schwarz, Ulrich S. (Author)
Format: Article (Journal)
Language:English
Published: 11 February 2010
In: Biophysical journal
Year: 2010, Volume: 98, Issue: 4, Pages: 543-551
ISSN:1542-0086
DOI:10.1016/j.bpj.2009.11.001
Online Access:Verlag, kostenfrei, Volltext: http://dx.doi.org/10.1016/j.bpj.2009.11.001
Verlag, kostenfrei, Volltext: http://www.sciencedirect.com/science/article/pii/S0006349509016890
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Author Notes:C. Selhuber-Unkel, T. Erdmann, M. López-García, H. Kessler, U.S. Schwarz, and J.P. Spatz
Description
Summary:Spatial patterning of biochemical cues on the micro- and nanometer scale controls numerous cellular processes such as spreading, adhesion, migration, and proliferation. Using force microscopy we show that the lateral spacing of individual integrin receptor-ligand bonds determines the strength of cell adhesion. For spacings ≥90 nm, focal contact formation was inhibited and the detachment forces as well as the stiffness of the cell body were significantly decreased compared to spacings ≤50 nm. Analyzing cell detachment at the subcellular level revealed that rupture forces of focal contacts increase with loading rate as predicted by a theoretical model for adhesion clusters. Furthermore, we show that the weak link between the intra- and extracellular space is at the intracellular side of a focal contact. Our results show that cells can amplify small differences in adhesive cues to large differences in cell adhesion strength.
Item Description:Gesehen am 08.12.2017
Physical Description:Online Resource
ISSN:1542-0086
DOI:10.1016/j.bpj.2009.11.001

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