In vitro analysis of nucleic acid recognition in B lymphocytes
In contrast to murine B cells, Toll-like receptor (TLR) expression in human B cells is mainly restricted to endosomally localized TLR7 and -9, receptors for RNA and DNA, respectively. Most importantly, B lymphocytes lack classical phagocytic receptors and instead internalize antigen only via the B c...
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| Main Authors: | , |
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| Format: | Chapter/Article |
| Language: | English |
| Published: |
31 May 2014
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| In: |
Innate DNA and RNA recognition
Year: 2014, Pages: 87-96 |
| DOI: | 10.1007/978-1-4939-0882-0_9 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1007/978-1-4939-0882-0_9
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| Author Notes: | Saskia Ziegler, Isabelle Bekeredjian-Ding |
| Summary: | In contrast to murine B cells, Toll-like receptor (TLR) expression in human B cells is mainly restricted to endosomally localized TLR7 and -9, receptors for RNA and DNA, respectively. Most importantly, B lymphocytes lack classical phagocytic receptors and instead internalize antigen only via the B cell receptor (BCR), a surface immunoglobulin specific for a defined antigen. BCR ligation triggers internalization of particulate antigens and physically associated molecules among them bacterial DNA or RNA. Thereby, this process provides access to endosomal nucleic acid-sensing TLRs. Co-stimulation of BCR and TLR ultimately leads to T cell-independent B cell activation. Here, we explain how this process can be experimentally mimicked in human peripheral blood B cells, e.g., using a microsphere-based system that promotes uptake of nucleic acid-based TLR ligands via BCR engagement. |
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| Item Description: | Gesehen am 24.02.2021 |
| Physical Description: | Online Resource |
| ISBN: | 9781493908820 |
| DOI: | 10.1007/978-1-4939-0882-0_9 |