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In vitro analysis of nucleic acid recognition in B lymphocytes

In contrast to murine B cells, Toll-like receptor (TLR) expression in human B cells is mainly restricted to endosomally localized TLR7 and -9, receptors for RNA and DNA, respectively. Most importantly, B lymphocytes lack classical phagocytic receptors and instead internalize antigen only via the B c...

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Bibliographic Details
Main Authors: Ziegler, Saskia (Author) , Bekeredjian-Ding, Isabelle (Author)
Format: Chapter/Article
Language:English
Published: 31 May 2014
In: Innate DNA and RNA recognition
Year: 2014, Pages: 87-96
DOI:10.1007/978-1-4939-0882-0_9
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1007/978-1-4939-0882-0_9
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Author Notes:Saskia Ziegler, Isabelle Bekeredjian-Ding

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520 |a In contrast to murine B cells, Toll-like receptor (TLR) expression in human B cells is mainly restricted to endosomally localized TLR7 and -9, receptors for RNA and DNA, respectively. Most importantly, B lymphocytes lack classical phagocytic receptors and instead internalize antigen only via the B cell receptor (BCR), a surface immunoglobulin specific for a defined antigen. BCR ligation triggers internalization of particulate antigens and physically associated molecules among them bacterial DNA or RNA. Thereby, this process provides access to endosomal nucleic acid-sensing TLRs. Co-stimulation of BCR and TLR ultimately leads to T cell-independent B cell activation. Here, we explain how this process can be experimentally mimicked in human peripheral blood B cells, e.g., using a microsphere-based system that promotes uptake of nucleic acid-based TLR ligands via BCR engagement. 
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